DIPA-CRISPR Archives - Astrobiology

Experimental scheme for DIPA-CRISPR in R. varieornatus. -- Representative photographs of R. varieornatus eggs (A) and individuals at 8 (B) and 10 days old (C). (D) The stage of parental females to be injected is important for successful gene editing in DIPA-CRISPR. To obtain parental females at the defined age, eggs were collected and their hatching was examined daily. Newly hatched juveniles (0 days old) were separated and reared for the defined period prior to injection. After the injection of Cas9 RNPs, the injected tardigrades were reared for 10 days, and the laid eggs (G0 progeny) were collected and reared. Grown G0 individuals were separately subjected to genomic DNA extraction and PCR. PCR amplicons were directly analyzed by Sanger sequencing. Gene-edited G0 progeny were obtained from parents injected at 7 to 10 days old. -- https://doi.org/10.1371/journal.pgen.1011298.g001

Cryobiology, Extremeophiles and Extreme Environments, Genomics, Proteomics, Bioinformatics, Microbiology & Virology, Nanotechnology & SynBio, Press Release, Radiation, Tardigrades

Modifying Genomes Of Tardigrades To Unravel Their Secrets

by Keith CowingJune 14, 2024June 14, 2024

Some species of tardigrades are highly and unusually resilient to various extreme conditions fatal to most other forms of life.