![Effects of filtration method and sequencing platform on microbial abundance and community composition. Comparison of microbial abundance, sequencing performance, and taxonomic composition among Yellowstone hot spring samples processed using Sterivex, Supor, and polycarbonate (PC) filtration devices and analyzed by Illumina and Oxford Nanopore sequencing. Upper panels show absolute microbial abundance estimates (black bars) and sequence read lengths (red symbols). Lower panels depict species-level relative abundances of dominant bacterial and archaeal (red asterisk) taxa recovered from each sample. -- [biorxiv.org]](https://i0.wp.com/astrobiology.com/wp-content/uploads/2026/07/Differential-efficiency.png?resize=1200%2C708&ssl=1)
[biorxiv.org] Metagenomic characterization of low-biomass Yellowstone National Park (YNP) hot spring waters remains challenging because microbial recovery is influenced by filtration methodology, sample preservation, DNA extraction, and sequencing strategy.
Extremeophiles and Extreme Environments
Posted inExtremeophiles and Extreme Environments
Rising From The Fragments: A Multi-factorial Regulatory Framework Of DNA Damage Response In Deinococcus radiodurans
[Extremophiles] Understanding the coordinated regulation of gene expression in response to environmental challenges is critical for unravelling the resistance strategies of the extremophiles that endure extreme habitats.
Posted inArctic / Antarctic / Alpine
Could Lake Joyce Have Acted As A Long-lived Proglacial Refugium In Antarctica’s McMurdo Dry Valleys?
[Arctic, Antarctic, and Alpine Research] Lake Joyce of the McMurdo Dry Valleys (MDV), Antarctica, contains an endemic copepod population (Diacyclops joycei) that has not been identified from apparently suitable habitats […]
![Deep-sea microbial sample processing. Detailed sample processing steps for HPG assimilating cells and total cell counts are denoted in grey and black arrows/squares, respectively. Processing steps and samples used for metagenomic sequencing are denoted in light-blue arrows and squares. “Whole sample” correspond to triplicate 50 ml aliquots, each filtered onto a 0.22 μm filter directly from the cryopreserved material, and then released with 200 μl of 0.025% (v/v) Tween 20 in PBS to be processed through MDA and DNA sequencing; “all cells” correspond to samples of cryopreserved material that were stained with a DNA dye, collected on a 0.22 μm filter, processed through the BONCAT labelling assay, and then sorted by flow cytometry, aiming to select events that presented the DNA dye; “BONCAT+” cell samples originated from the same material as ‘all cells’, but their sorting aimed for events that presented both the DNA dye and the fluorescent dye Alexa-Fluor 488 (attached to the HPG incorporated by the active cells). The selection of BONCAT+ cells was established for each sample based on the level of BONCAT background labelling to the microbial community when not exposed to HPG, or when fixed before being exposed to HPG (i.e. reflecting the adhesion of the fluorescent label to cells that did not incorporate HPG). -- [ISME Journal via PubMed]](https://i0.wp.com/astrobiology.com/wp-content/uploads/2026/07/Sample-Return.png?resize=1200%2C719&ssl=1)
Posted inSample Return
Sample Return: Minimizing Decompression And Warming During Deep Seawater Collection Increases Abundance And Activity Of Autochthonous Bacteria And Archaea
[ISME Journal via PubMed] The deep ocean hosts autochthonous pressure-adapted microorganisms that are unique to this environment, as well as allochthonous pressure-sensitive members transported from shallow depths by vertical advection […]
Posted inExtremeophiles and Extreme Environments
Sample Return From Earth’s Deep Biosphere
[Northwestern University] By studying life deep inside a former gold mine, a Northwestern University-led team of scientists uncovered evidence that Earth’s hidden biosphere operates less like a random collection of […]
Posted inTitan
Dragonfly Titan Astrobiology Droid Clears Key Tests
The mission reached a major milestone on June 29, when the Dragonfly team at the Johns Hopkins Applied Physics Laboratory (APL) in Laurel, Maryland, delivered the nearly 13-foot-long fuselage for […]
Posted inAway Teams & Field Reports
Expedition Led By MBARI Researchers Reveals A Remarkable, And Rare, Form Of Deep-sea Hydrothermal Vents
[MBARI] An international team of researchers, led by MBARI Senior Scientist Aaron Micallef and Schmidt Ocean Institute, has discovered two remarkable fields of hydrothermal vents in the Doldrums Megatransform and […]
Posted inTricorders & Sensors
Tricorder Tech: Identifying Proteins, Molecule By Molecule
[Université de Genève] Proteins are responsible for most functions in the human body. However, their analysis, which is essential for understanding diseases, developing drugs, and discovering new biomarkers, remains highly […]
Posted inAway Teams & Field Reports
Ocean Planet Research: 31 New Species Discovered in Two Weeks of Deep Sea Exploration
[Schmidt Ocean Institute] An international team of midwater experts on board Schmidt Ocean Institute’s research vessel Falkor (too) discovered over two dozen new marine species on a recent expedition off […]
Posted inAnalog Studies, Away Teams & Field Reports, Biochemistry & Organic Chemistry, Biogeochemical Cycles & Geobiology, Biosignatures & Paleobiology, Desert Worlds, Exploration Gear & Tech, Extremeophiles and Extreme Environments, Genomics, Proteomics, Bioinformatics, Mars, Microbiology & Virology, Sample Return
Lipid Biomarkers In Microbialites From A Maar Lake: An Astrobiological Study Of Biomarker Preservation under Mars Analog Conditions
Lipids constitute resistant biomarkers that have been proposed as pacemakers in the search for possible microbial life-forms in Mars’ past.