![Rapid coalescence and RNA exchange between unstable protocells. (A) Schematic of preparation of coacervate model protocells. Aqueous solutions of PDDA (positively charged) and ATP (negatively charged) are mixed, along with fluorescently labeled protein or oligonucleotide molecules, to make fluorescently distinct protocell populations. (B and C) Confocal micrographs showing the coalescence of unstable protocells during mixing. Fluorescently distinct PDDA-ATP droplet populations containing fluorophore-labeled BSA or RNA were mixed, and the images were collected after 5 min. Coalescence is evident from the overlapping of red [CF488-BSA in (B) or Cy2-RNA-6nt in (C)] and blue (CF640-BSA) fluorescence in a majority of the droplets. Scale bars, 25 μm. (D and E) Confocal micrographs and the accompanying FRAP plot showing a fast (timescale of a few minutes) recovery of fluorescence from Cy2-labeled RNA of (D) 6 nt in length and (E) 49 nt in length in the photobleached droplets, suggesting a rapid exchange of RNA molecules between protocells. The fluorescence microscopy images are false colored for clarity. Scale bars, 25 μm. -- Science / UChicago](https://i0.wp.com/astrobiology.com/wp-content/uploads/2024/08/New-Research-Suggests-Rainwater.png?resize=1200%2C719&ssl=1)
One of the major unanswered questions about the origin of life is how droplets of RNA floating around the primordial soup turned into the membrane-protected packets of life we call […]
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