electroporation Archives

EOD exposure from electric eel to zebrafish larvae. (A) This illustration depicts the experimental tank used to expose the recipient organism to the electric eel’s electric organ discharge (EOD). Within the tank, three carbon rod electrodes are placed: two inputs (colored black and red) and a ground electrode (colored green). (B) An EOD is induced by the electric eel during its predatory behavior when it feeds on a goldfish. The cuvette containing zebrafish larvae and a DNA solution is positioned in close proximity to the high-voltage pulses generated by the electric eel. The magenta curve illustrates the electric field produced by the electric eel. (C) This illustration indicates the construction of the GFP expression plasmid driven by the Oryzias latipes actb promoter. (D) The photograph displays 7-day post-fertilization (dpf) Danio rerio (zebrafish) larvae that were subjected to microinjection with the indicator plasmid at the one-to-eight cell stages. The plasmid has resulted in robust and widespread GFP fluorescence without apparent developmental abnormalities. Scale bar, 500 µm. (E) An example of a single predatory behavior with the EOD pulses lasting 30 s is shown. The electric eel first bites and swallows the goldfish (indicated by the magenta arrow, 1st bite), followed by another bite at an empty clip (indicated by the blue arrow, 2nd bite). -- Nagoya University

Electricity From Electric Eels May Transfer Genetic Material To Nearby Animals

by Keith CowingDecember 6, 2023December 6, 2023

Editor’s note: as is stated in this press release, electroporation is a well-known way to deliver genetic information into a cell – in the laboratory. The fact that this happens […]

electroporation

Electricity From Electric Eels May Transfer Genetic Material To Nearby Animals

NASA Establishes Mars Forward Operational Risk and Contamination Evaluation (M-FORCE) Tiger Team to Shape Mars Planetary Protection Protocols

Opportunity to Contribute to NASA DARES Community White Paper on Phototrophic Biosignatures

The Search For Life Elsewhere: Avoiding Hype and Fostering Public Understanding

Panchromatic Spectra of Nearby Low-mass and Sun-like Stars with Directly Imageable Habitable Zones

Alfred: A Flexible, User-Friendly Python Package for Exoplanet Confirmation