[q-bio.PE] Bacteriophage Qβ, first isolated in 1961, has served for more than six decades as a model system for molecular and viral evolution.
The development of cell-free replication systems based on the Qβ replicase enabled the first demonstrations of Darwinian evolution in RNA molecules. In parallel, the phage’s compact RNA genome, high mutation rate, and ease of propagation established Qβ as an exceptionally tractable model for studying viral evolution.
The discovery of extensive genetic heterogeneity made of Qβ the first biological system in which molecular quasispecies theory found direct experimental support. Building on this foundation, decades of evolution experiments under thermal, ecological, and mutagenic pressures established how mutation, selection, epistasis, and population structure jointly shape adaptive trajectories.
Deep sequencing has recently allowed the mutant spectrum of Qβ populations to be resolved as large, structured genotype networks, revealing a hierarchical organization around dominant sequences that generalizes classical mutation-selection balance to a dynamic process, and uncovering topological features shared with RNA viruses evolving within natural hosts.
In parallel, the catalytic core of the Qβ replication system has been repurposed in synthetic biology to revive cell-free Darwinian evolution within compartmentalized, cell-like systems, opening new avenues for studying the origin and early evolution of life. Further recent work has pushed the phage into new experimental territories, including extreme, astrobiologically relevant environments and biotechnological platforms for peptide display.
Together, these developments illustrate how a single bacteriophage has repeatedly anticipated and continues to clarify general principles of molecular, population, and ecological evolution.
Ester Lázaro, Susanna Manrubia
Comments: Review. 21 pages, 1 figure, 1 table
Subjects: Populations and Evolution (q-bio.PE)
Cite as: arXiv:2609.35594 [q-bio.PE] (or arXiv:2609.35594v1 [q-bio.PE] for this version)
https://doi.org/10.48550/arXiv.2609.35594
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Submission history
From: Susanna Manrubia Dr
[v1] Mon, 28 Sep 2026 16:44:09 UTC (546 KB)
https://arxiv.org/abs/2609.35594
Astrobiology, Genomics, Evolution,
