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a, Working model of rereading. b, Top box shows example trace of PASTOR-reread showing three almost-complete reread events (blue trace). Our model’s predicted signal for the PASTOR-reread sequence (pink trace) was aligned to each reread. The fourth VR contains an asparagine mutation, but the corresponding signal level consistently resembles aspartate in all three instances of this PASTOR-reread trace. The modelled sequence was changed to contain an aspartate to reflect the putative PTM. Bottom box shows the approximate region of the strand that is in the nanopore over time. -- Nature

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Tricorder Tech: Multi-pass, Single-molecule Nanopore Reading of Long Protein Strands

by Keith CowingNovember 27, 2024November 27, 2024

Editor’s note: Oxford Nanopore technology has been used multiple times on the International Space Station (see Genomics, Proteomics, Bioinformatics) to try and help elucidate changes in genomic function during exposure […]

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Tricorder Tech: Multi-pass, Single-molecule Nanopore Reading of Long Protein Strands

Dinosaur-killing Asteroid Also Created A Dust Cloud That Insulated Earth, Charbroiling Cretaceous Creatures

Current Water Trapping Micro-habitats On The Surface Of Mars

Methanotrophy Under Extreme Alkalinity In A Serpentinizing System

ANDES, The High-resolution Spectrograph Oof The ELT: Simulated Performance Of The CORO Module And Overview Of The High-contrast Capabilities For Exoplanet Observations

When Polymers Meet Primitive Membranes: How Molecular Cooperation May Have Helped Life Begin