E. coli REL606 Archives - Astrobiology

Schematic of project design. A E. coli REL606 strains were grown in High Aspect Ratio Vessels (HARV) on Rotating Wall Vessels (RWV) for simulated microgravity (SµG) and rotating (R) control in addition to immobile static (S) control. Each growth condition was assayed in glucose-limited (DM25) and glucose-replete (DM2000) media. B In a short-term culture study, the growth analysis of SµG, R, and S cultures in both media types was conducted. C Also in this short-term study, samples were collected at time of inoculation (T0), at 4.5 h post inoculation in DM25 and 6.5 h post inoculation in DM2000 (T1), and 24 h post inoculation (T2) for each condition for RNAseq and differential gene expression analysis. D In a follow-on long-term culture study, samples were passaged each day for 28 days. Samples from every 7th day of culture were subjected to whole genome sequencing to assay the genomic alterations for each growth condition. Created with BioRender.com -- BMC Microbiology

Extremeophiles and Extreme Environments, Gravitational Biology, Microbiology & Virology, Microgravity, Space Life Science, Space Stations, Status Report

Simulated Microgravity Triggers A Membrane Adaptation To Stress In E. coli REL606

by Keith CowingJuly 17, 2025July 17, 2025

Investigating the evolution of Escherichia coli in microgravity offers valuable insights into microbial adaptation to extreme environments.