auxotrophic Archives

Journey of a noncanonical amino acid into a polypeptide. The cell takes up the ncAA from its environment or biosynthesizes it. The ncAA should not interfere with the host metabolism nor act as a toxin. To accumulate, it must not be degraded nor excreted by the cell. If its intracellular concentration reaches an adequate level, an aminoacyl-tRNA synthetase (AARS) activates it and charges it onto a tRNA. Discrimination against the ncAA by the editing function of some AARS prevents the downstream steps and should be avoided. An elongation factor delivers the ncAA-charged tRNA to the ribosome, where it participates in ribosomal translation. Peptide bonding stably incorporates the ncAA into the nascent polypeptide. The resulting alloprotein must be able to fold correctly and should not be degraded, e.g., by proteolytic enzymes. Chemical Reviews via PubMed

Astropharmacy / Biotechnology, Biochemistry & Organic Chemistry, Biophysics, Biosignatures & Paleobiology, Commercial & Private Sector, Genomics, Proteomics, Bioinformatics, Nanotechnology & SynBio, Press Release

Residue-Specific Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts: Quo Vadis?

by Keith CowingSeptember 25, 2025September 25, 2025

The residue-specific incorporation of noncanonical amino acids in auxotrophic hosts allows the global exchange of a canonical amino acid with its noncanonical analog.

auxotrophic

Residue-Specific Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts: Quo Vadis?

ALMA Central Molecular Zone Exploration Survey (ACES) I: Overview

RNA And Proteins Joined Up At The Origins Of Life: Persistence Is The Point

Selection For Growth Drives The Emergence Of Genetic Heredity In Protocells

NASA SMD ROSES-25 Amendment 65: New Opportunity: C.4 Planetary Science Enabling Facilities

TESS Photometry and Radial Velocity Analysis of the sub-Neptune Exoplanet π Mensae c and the Wider π Mensae Planetary System