[ISME Journal via PubMed] The deep ocean hosts autochthonous pressure-adapted microorganisms that are unique to this environment, as well as allochthonous pressure-sensitive members transported from shallow depths by vertical advection […]
![Deep-sea microbial sample processing. Detailed sample processing steps for HPG assimilating cells and total cell counts are denoted in grey and black arrows/squares, respectively. Processing steps and samples used for metagenomic sequencing are denoted in light-blue arrows and squares. “Whole sample” correspond to triplicate 50 ml aliquots, each filtered onto a 0.22 μm filter directly from the cryopreserved material, and then released with 200 μl of 0.025% (v/v) Tween 20 in PBS to be processed through MDA and DNA sequencing; “all cells” correspond to samples of cryopreserved material that were stained with a DNA dye, collected on a 0.22 μm filter, processed through the BONCAT labelling assay, and then sorted by flow cytometry, aiming to select events that presented the DNA dye; “BONCAT+” cell samples originated from the same material as ‘all cells’, but their sorting aimed for events that presented both the DNA dye and the fluorescent dye Alexa-Fluor 488 (attached to the HPG incorporated by the active cells). The selection of BONCAT+ cells was established for each sample based on the level of BONCAT background labelling to the microbial community when not exposed to HPG, or when fixed before being exposed to HPG (i.e. reflecting the adhesion of the fluorescent label to cells that did not incorporate HPG). -- [ISME Journal via PubMed]](https://i0.wp.com/astrobiology.com/wp-content/uploads/2026/07/Sample-Return.png?resize=1200%2C719&ssl=1)
[ISME Journal via PubMed] The deep ocean hosts autochthonous pressure-adapted microorganisms that are unique to this environment, as well as allochthonous pressure-sensitive members transported from shallow depths by vertical advection […]